Influence of Lightpipes on Readers for Microtubes Fluorescence Detection

Eduardo de Araujo Rocha; Clara Mariah Loureiro da Silva; Valmara Silveira Ponte; Valéria Loureiro da Silva; Vinicius Pinto Rocha; Milena Botelho Pereira Soares

doi:10.34178/jbth.v5i4.240

Eduardo de Araujo Rocha
Clara Mariah Loureiro da Silva
Valmara Silveira Ponte
Valéria Loureiro da Silva
Vinicius Pinto Rocha
Milena Botelho Pereira Soares

DOI: https://doi.org/10.34178/jbth.v5i4.240

Resumo

Lightpipes are light guides based on total internal reflection used for LED illumination systems with many applications. This work investigates using lightpipes in a fluorescent reader for microtubes. It is part of a project to develop an RT-RPA multiplex test and fluorescence reader for SARS-COV 2 and H1N1. We investigated the lightpipes interaction with an excitation filter. The results do not show a benefit in using them on the emission side due to the capture of LED scattered light.