Bioprospecting Bacteria with Cellulolytic Potential in Cocoa Husks

Abstract

This study aimed to identify bacteria with cellulolytic degradation potential and evaluate their ability to degrade cellulose. Samples of cocoa fruit were collected, treated, and inoculated into a carboxymethylcellulose (CMC) medium, with cellulose as the sole carbon source. After microbial growth, the bacteria were isolated, morphologically characterized, and assessed for their cellulolytic activity. A total of 42 colonies were observed on the initial four plates. The results were promising, with the largest degradation halo measuring approximately 46 mm and an enzymatic index of 3.4. These findings highlight the biotechnological relevance of this research and its contribution to ongoing studies in the field.