Potential Application of Bacterial Lipase from Bacillus subtilis in the Production of Biodisel

Gabriele Marques dos Santos; Davi de Freitas Schuenemann; João Pedro Silva Santana; Fábio Alexandre Chinalia; Tatiana Oliveira do Vale

doi:10.34178/jbth.v6i4.329

Gabriele Marques dos Santos
Davi de Freitas Schuenemann
João Pedro Silva Santana
Fábio Alexandre Chinalia
Tatiana Oliveira do Vale

DOI: https://doi.org/10.34178/jbth.v6i4.329

Keywords: Bacillus subtilis, Lipase, Biodiesel, Immobilization

Abstract

This work aims to evaluate the potential of bacterial lipase of Bacillus subtilis in biodiesel production. Assays were performed to obtain, purify, and immobilize the enzyme and compare chemical and enzymatic transesterification tests. The Tryptic Soy Broth (TSB) culture medium without supplementation showed the highest enzymatic activity and purification yields when using 60% of (NH4)2SO4. Citral was the most efficient agent for lipase immobilization. Even though the free enzymes showed a higher activity rate, the immobilized enzymes are associated with a better-quality product. In conclusion, the lipase enzyme showed positive potential in biodiesel production.